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Nacalai protease inhibitor cocktail mix
Protease Inhibitor Cocktail Mix, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+inhibitor+mixture/cocktail+inhibitor+protease/us12622891-872-11-15
Average 86 stars, based on 1 article reviews
protease inhibitor cocktail mix - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Lysis:

Article Title: Lipid flippases ATP9A and ATP9B form a complex and contribute to the exocytic pathway from the Golgi.
Article Snippet: Immunoblots were developed using ImmunoStar (Wako) and recorded on an ImageQuant 800 instrument (Amersham). .. Cells were lysed in a lysis buffer (20 mM Hepes, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA) with a protease inhibitor mixture (Nacalai Tesque) at 4°C for 60 min. ..

Article Title: miR-199a functions downstream of MeCP2 in neurons of MECP2 duplication syndrome models
Article Snippet: .. The cell extracts were isolated by first washing the cells with cold PBS followed by lysis with NP40 buffer (Invitrogen) containing 1% protease inhibitor mixture (Nacalai) and 1% phosphatase inhibitor mixture (Nacalai). .. Lysates were centrifuged at 20,000 x g for 15 min. Total cell lysates were subjected to SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA) using Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA).

Article Title: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding
Article Snippet: .. After centrifugation, the TGC and sperm were homogenized in the cell lysis buffer containing protease inhibitor mixture (Nacalai Tesque, Japan) or boiled in the sample buffer. ..

Article Title: The eukaryotic elongation factor 2 kinase inhibitor, A484954, induces hypoglycaemic and hypotensive effects.
Article Snippet: Funding information Japan Society for the Promotion of Science (JSPS) KAKENHI, Grant/Award Number: 22K20617; Kitasato University Research Grant for Young Researchers; Kitasato University Research Grant Background and Purpose: Eukaryotic elongation factor 2 kinase (eEF2K) belongs to the Ca/calmodulin-dependent protein kinase family.. We previously revealed that A484954, a selective eEF2K inhibitor, caused hypotensive and diuretic effects via the production of nitric oxide (NO) in spontaneously hypertensive rats.. Otsuka Long– Evans Tokushima Fatty (OLETF) rats are hypertensive because of obesity and type 2 diabetes.

Article Title: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding.
Article Snippet: .. After centrifugation, the TGC and sperm were homogenized in the cell lysis buffer containing protease inhibitor mixture (Nacalai Tesque, Japan) or boiled in the sample buffer. ..

Protease Inhibitor:

Article Title: Lipid flippases ATP9A and ATP9B form a complex and contribute to the exocytic pathway from the Golgi.
Article Snippet: Immunoblots were developed using ImmunoStar (Wako) and recorded on an ImageQuant 800 instrument (Amersham). .. Cells were lysed in a lysis buffer (20 mM Hepes, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA) with a protease inhibitor mixture (Nacalai Tesque) at 4°C for 60 min. ..

Article Title: miR-199a functions downstream of MeCP2 in neurons of MECP2 duplication syndrome models
Article Snippet: .. The cell extracts were isolated by first washing the cells with cold PBS followed by lysis with NP40 buffer (Invitrogen) containing 1% protease inhibitor mixture (Nacalai) and 1% phosphatase inhibitor mixture (Nacalai). .. Lysates were centrifuged at 20,000 x g for 15 min. Total cell lysates were subjected to SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA) using Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA).

Article Title: TEX30 safeguards sperm morphogenesis and quality consistency required for male fertility.
Article Snippet: .. After centrifugation, 2 these samples were homogenized in TBS−Triton X−100 (137 mM NaCl, 2.7 mM KCl, 25 mM 3 Tris, 1% Triton X−100, pH 7.4) containing protease inhibitor mixture (Nacalai Tesque, Kyoto, 4 Japan). ..

Article Title: FABP4-dependent fatty acid oxidation-fueled mitochondrial ROS induces the mobilization of cellular iron and facilitates Trypanosoma cruzi proliferation in murine adipocytes.
Article Snippet: .. Cells were washed once with ice-cold PBS, lysed in RIPA buffer with protease inhibitor mixture (NACALAI TESQUE, INC., Japan), and then briefly sonicated. .. Membranes were blocked with Blocking One (NACALAI TESQUE, INC.) for 30 min and incubated overnight at 4°C with primary Ab diluted in Can Get Signal solution 1 (TOYOBO).

Article Title: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding
Article Snippet: .. After centrifugation, the TGC and sperm were homogenized in the cell lysis buffer containing protease inhibitor mixture (Nacalai Tesque, Japan) or boiled in the sample buffer. ..

Article Title: miR-199a functions downstream of MeCP2 in neurons of MECP2 duplication syndrome models
Article Snippet: NP40 buffer , Invitrogen , Cat#FNN0021. .. Protease inhibitor mixture , Nacalai , Cat#03969-21. .. Phosphatase inhibitor mixture , Nacalai , Cat#07575-51.

Article Title: The eukaryotic elongation factor 2 kinase inhibitor, A484954, induces hypoglycaemic and hypotensive effects.
Article Snippet: Funding information Japan Society for the Promotion of Science (JSPS) KAKENHI, Grant/Award Number: 22K20617; Kitasato University Research Grant for Young Researchers; Kitasato University Research Grant Background and Purpose: Eukaryotic elongation factor 2 kinase (eEF2K) belongs to the Ca/calmodulin-dependent protein kinase family.. We previously revealed that A484954, a selective eEF2K inhibitor, caused hypotensive and diuretic effects via the production of nitric oxide (NO) in spontaneously hypertensive rats.. Otsuka Long– Evans Tokushima Fatty (OLETF) rats are hypertensive because of obesity and type 2 diabetes.

Article Title: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding.
Article Snippet: .. After centrifugation, the TGC and sperm were homogenized in the cell lysis buffer containing protease inhibitor mixture (Nacalai Tesque, Japan) or boiled in the sample buffer. ..

Isolation:

Article Title: miR-199a functions downstream of MeCP2 in neurons of MECP2 duplication syndrome models
Article Snippet: .. The cell extracts were isolated by first washing the cells with cold PBS followed by lysis with NP40 buffer (Invitrogen) containing 1% protease inhibitor mixture (Nacalai) and 1% phosphatase inhibitor mixture (Nacalai). .. Lysates were centrifuged at 20,000 x g for 15 min. Total cell lysates were subjected to SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA) using Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA).

Centrifugation:

Article Title: TEX30 safeguards sperm morphogenesis and quality consistency required for male fertility.
Article Snippet: .. After centrifugation, 2 these samples were homogenized in TBS−Triton X−100 (137 mM NaCl, 2.7 mM KCl, 25 mM 3 Tris, 1% Triton X−100, pH 7.4) containing protease inhibitor mixture (Nacalai Tesque, Kyoto, 4 Japan). ..

Article Title: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding
Article Snippet: .. After centrifugation, the TGC and sperm were homogenized in the cell lysis buffer containing protease inhibitor mixture (Nacalai Tesque, Japan) or boiled in the sample buffer. ..

Article Title: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding.
Article Snippet: .. After centrifugation, the TGC and sperm were homogenized in the cell lysis buffer containing protease inhibitor mixture (Nacalai Tesque, Japan) or boiled in the sample buffer. ..

Sonication:

Article Title: FABP4-dependent fatty acid oxidation-fueled mitochondrial ROS induces the mobilization of cellular iron and facilitates Trypanosoma cruzi proliferation in murine adipocytes.
Article Snippet: .. Cells were washed once with ice-cold PBS, lysed in RIPA buffer with protease inhibitor mixture (NACALAI TESQUE, INC., Japan), and then briefly sonicated. .. Membranes were blocked with Blocking One (NACALAI TESQUE, INC.) for 30 min and incubated overnight at 4°C with primary Ab diluted in Can Get Signal solution 1 (TOYOBO).



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